SMART-Seq® mRNA Single Cell LP
Cat# 634786
Price $1,196 / each
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SMART-Seq® mRNA LP
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Cat. # 634768
Complete oligo(dT)-primed, full-length mRNA-seq workflow for 1–1,000 cells or 10 pg–100 ng total RNA; Illumina-ready libraries in a single day.
Product overview
- Full-length coverage with SMART template switching
- Validated for ultra-low RNA and single-cell inputs
- Complete cDNA-to-library workflow in one kit
- Compatible with standard Illumina sequencers
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Why this product
10 pg–100 ng
Total RNA input
1–1,000 cells
Validated cell range
~7 hours
Sample to library
QC verified
CoA available
Overview
SMART-Seq mRNA LP generates full-length, strand-agnostic cDNA from ultra-low RNA inputs and converts it into Illumina-ready libraries in one validated workflow.
- Full-length transcript coverage — oligo(dT) priming with SMART (Switching Mechanism at 5' end of RNA Template) technology for even coverage across the transcript
- Ultra-low input flexibility — validated for 1–1,000 intact cells or 10 pg–100 ng of high-quality total RNA
- One complete workflow — cDNA synthesis, amplification and Illumina-compatible library preparation in a single kit
- Fewer handling steps — single-tube reverse transcription and template switching reduce sample loss
- Ready for expression and isoform analysis — supports differential expression, splice variants and SNP detection
- Sequencer-ready in one day — libraries can be completed within a standard working day
Specifications
Technical details for the selected 24 Rxns configuration.
| Catalog number | 634768 |
|---|---|
| Technology | SMART full-length cDNA synthesis (template switching) |
| Priming | Oligo(dT) |
| Input range | 1–1,000 cells or 10 pg–100 ng total RNA |
| Recommended RNA quality | RIN ≥ 8 for purified total RNA |
| Library output | Illumina-compatible dual-indexed libraries |
| Reactions per kit | 24, 96 or 384 |
| Hands-on time | ~4.5 hr for 24 samples |
| Storage | −20°C |
Values are representative; confirm the approved product documentation.
Required products
| Cat # | Product name | Description | Size | Price | Add |
|---|---|---|---|---|---|
| 634773 | SMART-Seq® Library Prep Index Kit | Dual-index primer set required to complete the library preparation portion of the SMART-Seq mRNA LP workflow. | 96 indexes | $420 | |
| 740609.10 | NucleoMag® Clean-up Beads | Magnetic beads used for cDNA and library clean-up steps described in the SMART-Seq mRNA LP protocol. | 5 ml | $180 |
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