01
In-Fusion® cloning
Design primers with confidence
Build single- or multiple-insert constructs, design site-directed mutagenesis primers, and move from sequence input to an export-ready protocol.
Best for
Single- or multiple-insert cloning · site-directed mutagenesis · vector linearization
Build your construct in four guided stages
Keep sequence input, primer decisions and export readiness visible in one workspace.
Your workflow
Primer design workspace
Choose a task — Clone, insert, delete or replace.
Learn by task
Short tutorials use visual examples so you can start with the workflow closest to your experiment.
02
Delete a sequence
3 min tutorial
03
Insert a sequence
4 min tutorial
04
Delete and replace
5 min tutorialBefore you start
Prepare vector and insert sequences in FASTA format. Keep the In-Fusion cloning region and intended junctions available.